Dublin City Council said it removed jackets that were hung on the Ha'Penny Bridge for health and safety reasons.
It comes after a video emerged on Saturday of a Dublin City Council worker removing jackets that were left on the bridge.
It was part of the 'Warm for Winter' campaign for the homeless.
The Inner City Helping Homeless charity had earlier criticised the removal of the coats.
Its head of communications, Brian McLoughlin said: "We would ask questions as to who in DCC authorised these jackets to be removed and where they have been taken.
"The campaign captured the attention of people and highlighted the need for supports for people that are homeless.
"Bad weather would obviously have impact on the campaign but I have spoken to Paddy Fryers, who founded the #warmforwinter campaign, and he confirmed that no one from DCC has made any effort to contact him before removing them.
"This matter also raises further questions on orders from the council relating to homelessness as our teams have been advised that the council have removed some tents on people while they were away getting something to eat.
"We would ask DCC to clarify why the jackets were removed and were they donated to a homeless charity or did they simply get rid of them."
In an apparent response on Twitter, Dublin City Council said: "Dublin City Council asks people not to hang clothes on the Ha'Penny Bridge for health & safety reasons.
Dublin City Council asks people not to hang clothes on the Ha’Penny Bridge for health & safety reasons- it reduces pedestrian flows & causes congestion on bridge.Please consider giving them to charity shops.Items collected by DCC will be redistributed via our homeless services
— Dublin City Council (@DubCityCouncil) December 7, 2019